The laminar burning velocity and Markstein length of n-tetradecane/air mixture were obtained under initial pressure of 0.1 MPa, initial temperatures of 420, 450 K and 480 K within a wide equivalence ratio range from 0.8 to 1.4 in a constant volume chamber, and the effects of initial temperature and equivalence ratio were analyzed. The results showed that the increase of initial temperature and equivalence ratio had little effect on the stability of the flame propagation images. Under the initial temperature of 480 K and equivalence ratio of 1.3, there was no crack or cellular structure in the flame. The effect that the increase of initial temperature can increase the flame propagation speed and promote the formation of flame front was observed, which was more significant in the lean mixture. With the increase of equivalence ratio, the Markstein length of n-tetradecane/air mixture decreased and the flame stability became worse. With the increase of temperature, the Markstein length decreased, and the unstretched flame speed and laminar burning velocity increased. In addition, compared with the laminar burning velocity of RP-3 kerosene, the laminar burning velocity of n-tetradecane was higher under most conditions.