The frosting and defrosting performance of the microtubule compact precooler was studied under the experimental conditions of the flow velocity, the humidity value and the temperature of the main flow of 10 m/s, 6.4 g/kg and 50 ℃, respectively. Under the defrosting experimental conditions, anhydrous methanol was utilized as the solvent for defrosting, and anhydrous methanol with three different mass ratios (0.75, 1.0 and 1.25) was sprayed into the main flow for defrost the precooler during the defrosting experiments. The frosting and defrosting performance, the pressure loss coefficient, the wall surface temperature of the precooler tube bundles, and the heat transfer rate of the precooler were analyzed in detail. The experimental results revealed that when the cryogenic coolant flowed through the inside of the microtubule bundles of the precooler, the frost later quickly condensed on the outside of the precooler, and the frost layer gradually accumulated with the experimental time. However, once anhydrous methanol of three different mass ratios was sprayed into the main flow, the defrosting effect was obviously improved, the pressure loss coefficient of the main flow was significantly decreased and the heat transfer rate of the precooler was evidently increased. In addition, in the defrosting experiments, the wall surface temperatures of the precooler tube bundles were also significantly increased higher than the freezing point of water, making the spraying anhydrous methanol produce defrosting effect. Among three different mass ratios of the sprayed anhydrous methanol, the best defrosting performance was obtained when the mass ratio of the sprayed anhydrous methanol was 1.0. Moreover, based on analysis of the defrosting experimental results, it can be further inferred that the optimal mass ratio to achieve the best defrosting performance may be between 1.0 and 1.25.